Detection and quantitation of a pheomelanin component in melanin pigments using pyrolysis-gas chromatography/tandem mass spectrometry system with multiple reaction monitoring mode |
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Authors: | Dzierżęga-Lęcznar Anna Kurkiewicz Slawomir Stępień Krystyna |
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Affiliation: | Department of Instrumental Analysis, Faculty of Pharmacy, Medical University of Silesia, Katowice, Poland. adler@sum.edu.pl |
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Abstract: | Here, we describe the reliable method for the detection and quantitation of a pheomelanin component in melanin pigments. Synthetic melanins with various contents of pheomelanin-type structural units were thermally degraded, and the multiple reaction monitoring mode was applied to detect the pheomelanin markers in the pyrolysates by GC/MS/MS. The method allowed the specific detection and quantitation of a pheomelanin component in melanin with the incorporation of pheomelanin-type units as low as 0.05%. Considering highly universal character of the pheomelanin markers, the method could be applied for structural studies of natural melanin pigments being mixtures of eumelanin and pheomelanin. |
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