| 石英晶体微天平的纳米微球质量放大技术及短序列DNA测定 |
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| 引用本文： | 张盛龙,彭图治.石英晶体微天平的纳米微球质量放大技术及短序列DNA测定[J].高等学校化学学报,2002,23(6):1022-1025. |
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| 作者姓名： | 张盛龙 彭图治 |
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| 作者单位： | 浙江大学西溪校区化学系, 杭州 310028 |
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| 基金项目： | 国家自然科学基金 (批准号 :2 9975 0 2 4)，浙江省自然科学基金 (批准号 :2 0 0 0 61)，分析测试基金资助 |
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| 摘 要： | 提出了一种新型质量放大系统用于提高石英晶体微天平检测的灵敏度.将DNA嵌入剂放线菌素D作为导向分子修饰在磁性微球上,通过石英晶体微天平技术识别测定短序列DNA,使最低检测限从6.2×10-8mol/L下降到2.0×10-12mol/L.
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| 关 键 词： | 石英晶体微天平 生物传感器 放线菌素D 磁性微球 自组单层膜 |
| 文章编号： | 0251-0790(2002)06-1022-04 |
| 收稿时间： | 2001-07-12 |
Microgravimetric Amplification and the Detection of Short DNA Sequences on a Quartz Crystal Microbalance by Modified Nano-microspheres |
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| ZHANG Sheng Long,PENG Tu Zhi.Microgravimetric Amplification and the Detection of Short DNA Sequences on a Quartz Crystal Microbalance by Modified Nano-microspheres[J].Chemical Research In Chinese Universities,2002,23(6):1022-1025. |
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| Authors: | ZHANG Sheng Long PENG Tu Zhi |
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| Institution: | Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China |
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| Abstract: | Anovel and sensitive microgravimetric biosensor has been developed by using actinomycin Dfunctionalized magnetic nano-microspheres, which can be intercalated in duplex strands DNAs on the gold electrode of a quartz crystal microbalance. These modified nano-microspheres have been used as the amplified label for microgravimetric signal owing to their heavy mass. The linear range for the determination of target DNAis from 10-9 to 10-11 mol/L, and the detection limit is found to be 2×10-12mol/L. |
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| Keywords: | Quartz crystal microbalance Biosensor Actinomycin D Magnetic microsphere Self assembly monolayer film |
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