Abzyme generation using an anti-idiotypic antibody as the “internal image” of an enzyme active site |
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Authors: | Alain Friboulet Ladan Izadyar Berangere Avalle Alberto Roseto Daniel Thomas |
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Affiliation: | (1) Laboratorie de Technologie Enzymatique, Universit’e de Technologie de Compiegne, BP 649, 60206 Compiegne Cedex, France;(2) Division d’Immunocytologie Appliqu’ee, URA 1442 du CNRS, Universit’e de Technologie de Compiegne, BP 649, 60206 Compiegne Cedex, France |
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Abstract: | Since the two reports published in 1986 by the laboratories of R. Lerner and P. G. Schultz, it has been clearly established that antibodies may be induced to act as catalysts in numerous chemical reactions. In all cases, catalytic antibodies were elicited using a substratebased approach. In the present article, we propose an alternative and complementary enzyme-based approach to generate catalytic antibodies. This approach uses the properties of anti-idiotypic antibodies to generate internal images of enzyme active sites. Experimental results are discussed for polyclonal and monoclonal anti-idiotypic antibodies. |
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Keywords: | Anti-idiotypes catalytic antibodies cholinesterase activity |
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