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Liquid chromatographic analysis of clenbuterol residues in food products of animal origin (cow liver) using diphasic dialysis extraction |
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| Authors: | P González Gigosos C A Fente Sampayo C Franco Abuín B Vázquez Belda E Quinto Fernández A Cepeda Sáez |
| Institution: | (1) Laboratorio de Salud Pública de Lugo, Consellería de Sanidade e Benestar Social, Xunta de Galicia, Lugo, Spain;(2) Laboratorio de Higiene e Inspección de Alimentos, Departamento de Química Analítica, Nutrición y Bromatología, Facultad de Veterinaria, Campus de Lugo, Universidad de Santiago de Compostela, Spain |
| Abstract: | Summary This paper reports a method employing diphasic dialysis extraction for the rapid confirmation of clenbuterol in cow liver by RPHPLC with diode-array detection. The most suitable means of homogenizing the liver is in barium hydroxide-barium chloride buffer; the optimum extraction solvent istert-butyl methyl ether at an extraction temperature of 37°C; stirring should be applied at 150 r.p.m. for 4h. This extraction method improves clenbuterol recovery values to 98.9%. |
| Keywords: | Reversed phase HPLC Diode-array detection Diphasic dialysis extraction Cow liver Clenbuterol |
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