| Photocycle of 13-cis-retinal in bacteriorhodopsin caused by destruction to the cooperative effect of purple membrane |
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| 引用本文： | 胡坤生,何靳安,张衡涛. Photocycle of 13-cis-retinal in bacteriorhodopsin caused by destruction to the cooperative effect of purple membrane[J]. 中国科学B辑(英文版), 1998, 0(2) |
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| 作者姓名： | 胡坤生 何靳安 张衡涛 |
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| 作者单位： | Department of Cell Biology，Institute of Biophysics，Chinese Academy of Sciences，Beijing 100101，China |
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| 基金项目： | ProjectsupportedbythekeyandmajorprojectsfromtheChineseAcademyofSciences (GrantNos.Kj95 1 Al 5 0 1 0 5andKj95 2 S1 0 3) |
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| 摘 要： | Ｉｎｔｅｇｒａｌｍｅｍｂｒａｎｅｐｒｏｔｅｉｎｓａｒｅｃｒｉｔｉｃａｌｔｏａｗｉｄｅｖａｒｉｅｔｙｏｆｃｅｌｌｕｌａｒｐｓｙｃｈｏｌｏｇｉｃａｌａｃｔｉｖｉｔｉｅｓ,ｓｕｃｈａｓｃｙｔｏｃｈｒｏｍｅｏｘｉｄａｓｅｉｎｍｅｄｉａｔｉｎｇｅｌｅｃｔｒｏｎｔｒａｎｓｐｏｒｔａｎｄｔｈｅａｃｔｉｖａｔｉｏｎｏｆｉｎｔｒａｃｅｌｌｕｌａｒｓｉｇｎａｌｓｂｙｔｒａｎｓｍｅｍｂｒａｎｅｒｅｃｅｐｔｏｒｓｃｏｕｐｌｅｄｔｏＧｐｒｏｔｅｉｎｓ[1].Ｉｎｔｈｅｓｅｍｅｍｂｒａｎｅａｃｔｉｖｉｔｉｅｓ,ｔｈｅｍｅｃｈａｎ…
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Photocycle of 13-cis-retinal in bacteriorhodopsin caused by destruction to the cooperative effect of purple membrane |
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| HU Kunsheng,HE Jin''''an and ZHANG Hengtao. Photocycle of 13-cis-retinal in bacteriorhodopsin caused by destruction to the cooperative effect of purple membrane[J]. Science in China(Chemistry), 1998, 0(2) |
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| Authors: | HU Kunsheng HE Jin''''an ZHANG Hengtao |
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| Abstract: | The steady absorption and kinetic changes of M 412 intermediate of the light and dark adapted bacteriorhodopsin (BR) solubilized by different concentrated Triton X 100 were investigated. The results indicated that the cooperative effect existing within the trimeric BR of native purple membrane (PM) was damaged in the system containing the surfactant since the component and structure of the bilayer lipid membrane in PM varied due to the solubilization of partial PM lipids by Triton X 100. The destruction to the cooperative effect of BR ultimately caused 13 cis retinal of the dark adapted BR to take part in BR photocycle and also to generate the deprotonated M 412 intermediate. |
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| Keywords: | bacteriorhodopsin purple membrane cooperative effect Triton X 100 M 412 intermediate. |
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