| 青霉素酰化酶在介孔分子筛MCM-41上的固定化研究 |
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| 引用本文： | 李晓芬,何静,马润宇. 青霉素酰化酶在介孔分子筛MCM-41上的固定化研究[J]. 化学学报, 2000, 58(2): 167-171 |
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| 作者姓名： | 李晓芬 何静 马润宇 |
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| 作者单位： | [1]北京化工大学应用化学系 [2]北京大学物理化学研究所 |
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| 摘 要： | 通过改变酶固定化时的pH值、温度、时间以及酶用量,研究了固定化条件对MCM-41载体上固定化青霉素酰化酶的酶活及其稳定性的影响,明确了酶活及其稳定性随固定化条件的变化规律,初步分析了青霉素酰化酶在介孔分子筛MCM-41上的固定化过程。
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| 关 键 词： | 青霉素酰化酶 分子筛 固定化 |
| 修稿时间： | 1999-03-15 |
The immobilization of penicillin G amidase on mesoporous molecular sieve MCM-41 |
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| LI Xiao-Fen,HE JingMA Run-YuDUAN XueZHU Yue-Xiang. The immobilization of penicillin G amidase on mesoporous molecular sieve MCM-41[J]. Acta Chimica Sinica, 2000, 58(2): 167-171 |
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| Authors: | LI Xiao-Fen HE JingMA Run-YuDUAN XueZHU Yue-Xiang |
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| Affiliation: | LI Xiao-Fen,HE Jing(Department of Applied Chemistry Beijing University of Chemical Technology, Beijing,100029)MA Run-Yu(Department of Chemical Engineering,Beijing University of Chemical Technology, Beijing,100029)DUAN Xue(Department of Applied Chemistry Beijing University of Chemical Technology, Beijing,100029)ZHU Yue-Xiang(The Institute of Physical Chemistry,Peking University,Beijing, 100029) |
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| Abstract: | Mesoporous molecular sieve MCM - 41 has been used as the support for the immobilization of penicillin G amidase(PGA) . The effects of immobilization conditions such as pH, temperature, time and ratio of PGA to MCM - 41 on the activity and stability of immobilized enzyme(IME) have been studied. It was found that the activity of ME decreased with the increase of pH, increased and then decreased with the increase of temperature and time, increased with the increase of the ratio of PGA to MCM - 41. It was also found that the stability of IME increased with the increase of temperature, increased and then decreased with the increase of time and the ratio of PGA to MCM - 41. Additionally, the process of PGA immobilized on MCM - 41 has been studied. |
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| Keywords: | MCM-41 penicillin G amidase activity of enzyme |
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