| 反向PCR扩增问号赖型钩体重组质粒pDJH2插入片段 |
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| 引用本文： | 刘洪斌,戴保民.反向PCR扩增问号赖型钩体重组质粒pDJH2插入片段[J].宁波大学学报(理工版),1999,12(3):66-68. |
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| 作者姓名： | 刘洪斌 戴保民 |
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| 作者单位： | 宁波大学医学院!浙江宁波３１５２１１(刘洪斌)，华西医科大学钩体病研究室!四川成都６１００４１(戴保民) |
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| 摘 要： | 钩端螺旋体赖型赖株（Ｌ．ｉｎｔｅｒｒｏｇａｎｓＳｅｒｏｖａｒｌａｉ）ＤＮＡ分别经ＢａｍＨＩ，ＨｉｎｄⅢ和ＰｓｔⅠ３种内切酶酶切完全消化，通过设计１对引物，反向ＰＣＲ扩增问号赖型钩体重组质粒ｐＤＪＨ２插入片段两端的未知序列．结果显示不同酶切环化连接，经反向ＰＣＲ扩增均可得２条大小相等的扩增带．结果提示反向ＰＣＲ技术可以用于问号赖型钩体重组质粒插入片段两端的未知序列的研究，为获得钩体完整基因序列提供新的途径．
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| 关 键 词： | 钩端螺旋体 反向PCR 重组质粒 |
Use of Inverse Polymerase Chain Reaction to Amplify DNA Adjacent to the Insert Fragment of pDJH_2 of L.Interrogans Serovar Lai |
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| LIU Hong-bin,DAI Bac-min.Use of Inverse Polymerase Chain Reaction to Amplify DNA Adjacent to the Insert Fragment of pDJH_2 of L.Interrogans Serovar Lai[J].Journal of Ningbo University(Natural Science and Engineering Edition),1999,12(3):66-68. |
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| Authors: | LIU Hong-bin DAI Bac-min |
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| Institution: | LIU Hong-bin1,DAI Bac-min2 |
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| Abstract: | DNA of L.interrogans Serovar lai was completely digested by Bam HI,Hind III and Pst I.respectively,then circularized by T4 ligase,and amplified by inverse PCR No matter whatever restrictionenzymes used, the amplification results were the same-two amplificahon bands(450 hp and 250 bP).Theresults indicate that inverse PCR can be used to study the DNA adjacent to a region of knownsequence and to play a role in searching for the complete gene of L.interrogans serovar lai. |
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| Keywords: | Leptospira Inverse PCR Recombinant plasmid |
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