Exploring Effects of Chitosan Oligosaccharides on the DSS-Induced Intestinal Barrier Impairment In Vitro and In Vivo |
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| Authors: | Yujie Wang Rong Wen Dongdong Liu Chen Zhang Zhuo A. Wang Yuguang Du |
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| Affiliation: | 1.State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; (Y.W.); (R.W.); (D.L.);2.School of Chemical Engineering, University of Chinese Academy of Sciences, Beijing 100049, China;3.College of Life Science, Sichuan Normal University, Chengdu 610101, China |
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| Abstract: | Intestinal barrier dysfunction is an essential pathological change in inflammatory bowel disease (IBD). The mucus layer and the intestinal epithelial tight junction act together to maintain barrier integrity. Studies showed that chitosan oligosaccharide (COS) had a positive effect on gut health, effectively protecting the intestinal barrier in IBD. However, these studies usually focused on its impact on the intestinal epithelial tight junction. The influence of COS on the intestinal mucus layer is still poorly understood. In this study, we explored the effect of COS on intestinal mucus in vitro using human colonic mucus-secreted HT-29 cells. COS relieved DSS (dextran sulfate sodium)-induced mucus defects. Additionally, the structural characteristics of COS greatly influenced this activity. Finally, we evaluated the protective effect of COS on intestinal barrier function in mice with DSS-induced colitis. The results indicated that COS could manipulate intestinal mucus production, which likely contributed to its intestinal protective effects. |
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| Keywords: | chitosan oligosaccharide intestinal barrier mucus intestinal tight junction inflammatory bowel disease |
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