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Direct Quantification of Nanoplastics Neurotoxicity by Single-Vesicle Electrochemistry |
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| Authors: | Shiyi Wei Prof Fei Wu Dr Jing Liu Dr Wenliang Ji Dr Xiulan He Ran Liu Prof Ping Yu Prof Lanqun Mao |
| Institution: | 1. Beijing National Laboratory for Molecular Science, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, No. 2 Zhongguancun North 1st St, Beijing, 100190 China
University of Chinese Academy of Sciences, No.1 Yanqihu East Rd, Beijing, 101408 China;2. College of Chemistry, Beijing Normal University, No. 19 Xinjiekouwai St, Beijing, 100875 China;3. Beijing National Laboratory for Molecular Science, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, No. 2 Zhongguancun North 1st St, Beijing, 100190 China Institute of Analysis and Testing, Beijing Academy of Science and Technology, No.27, West 3rd Ring North Rd, Beijing, 100089 China;4. Beijing National Laboratory for Molecular Science, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, No. 2 Zhongguancun North 1st St, Beijing, 100190 China |
| Abstract: | Nanoplastics are recently recognized as neurotoxic factors for the nervous systems. However, whether and how they affect vesicle chemistry (i.e., vesicular catecholamine content and exocytosis) remains unclear. This study offers the first direct evidence for the nanoplastics-induced neurotoxicity by single-vesicle electrochemistry. We observe the cellular uptake of polystyrene (PS) nanoplastics into model neuronal cells and mouse primary neurons, leading to cell viability loss depending on nanoplastics exposure time and concentration. By using single-vesicle electrochemistry, we find the reductions in the vesicular catecholamine content, the frequency of stimulated exocytotic spikes, the neurotransmitter release amount of single exocytotic event, and the membrane-vesicle fusion pore opening-closing speed. Mechanistic investigations suggest that PS nanoplastics can cause disruption of filamentous actin (F-actin) assemblies at cytomembrane zones and change the kinetic patterns of vesicle exocytosis. Our finding shapes the first quantitative picture of neurotoxicity induced by high-concentration nanoplastics exposure at a single-cell level. |
| Keywords: | Nanoplastics Neurotoxicity Neurotransmitter Secretion Single-Vesicle Electrochemistry Vesicle Exocytosis |