Quantitation of the benzodiazepine antagonist flumazenil in human plasma by gas chromatography-mass spectrometry |
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Authors: | E K Fukuda N Choma P P Davis |
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Institution: | Department of Drug Metabolism, Hoffmann-La Roche Inc., Nutley, NJ 07110. |
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Abstract: | A gas chromatographic-mass spectrometric procedure has been developed for the quantitation of the benzodiazepine antagonist flumazenil in human plasma. The assay utilizes an extraction at alkaline pH with benzene-dichloroethane (80:20), selective ion monitoring, isobutane positive-ion chemical ionization mass spectrometry and stable isotope dilution. The method has been used to measure plasma concentrations of flumazenil in over 1500 clinical samples over a range of 0.5-200 ng/ml (using 2 ml of plasma). |
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