Determination of the van der Waals, electron donor and electron acceptor surface tension components of static Gram-positive microbial biofilms |
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Authors: | Briandet R Herry J -M Bellon-Fontaine M -N |
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Institution: | a Institut National de la Recherche Agronomique, Unité de Recherche en Bioadhésion et Hygiène des Matériaux, 25 Avenue de la République, 91300 Massy, France b SKW Biosystems, Cultures & Enzymes Department, La Ferté-sous-Jouarre, France |
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Abstract: | A large number of studies have shown the influence of the physico-chemical properties of a surface on microbial adhesion phenomenon. In this study, we considered that the presence of a bacterial biofilm may be regarded as a “conditioning film” that may modify the physico-chemical characteristics of the support, and thus the adhesion capability of planktonic micro-organisms coming into contact with this substratum. In this context, we adapted a protocol for biofilm formation that allows, under our experimental conditions, contact angle measurements, the reference method to determine the energetic surface properties of a substratum. This made it possible to determine the van der Waals, electron acceptor and electron donor properties of static biofilms grown at 25°C on stainless-steel slides with six Gram-positive bacteria isolated in dairy plants. A variance analysis indicated significant effects (P<0.05) of the bacterial strains and of the physiological state of the micro-organisms (planktonic or sessile) on the contact angles. To link the energetic properties of the six biofilms with direct adhesion experiments, we measured the affinity of fluorescent carboxylate-modified polystyrene beads for the different biofilm surfaces. The results correlated best with the electron-acceptor components of the biofilm surface energies, stressing the importance of Lewis acid–base interactions in adhesion mechanisms. |
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Keywords: | Adhesion Biofilm Gram-positive bacteria Physico-chemical interactions |
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