稀土穴状荧光配合物的合成与光谱性质

以2,6-二甲基吡啶-3,5-二甲酸二乙酯为起始原料,经N-溴代丁二酰亚胺(NBS)溴代、亲电取代反应合成了时间分辨荧光免疫分析双功能螯合剂2,6-{N,N′,N,N′-二(2,2′-联吡啶-6,6′-二甲基)]二(氨甲基)}-吡啶-3,5-二羧酸二乙酯。经差热分析仪(DTA)、傅里叶变换红外光谱仪(FTIR)、核磁共振波谱仪(1 H NMR)、质谱仪(MS)等技术手段表征确认了化合物结构和性能。对该化合物与铕离子形成螯合物的荧光性质研究表明:激发光谱波长范围较宽,激发峰值为322nm;荧光发射峰为597nm(~5D_0-~7F_1)、618nm(~5D_0-~7F_2);荧光寿命为918μs;量子产率Φ=0.249。

Synthesis and Spectroscopic Properties of a Cryptate Rare Earth Fluorescence Complex

A bifuntional cryptate chelate 2,6-{N,N',N,N'-bis(2,2'-bipyridine-6,6'-dimethyl)]bis(aminomethyl)}-pyridine-3,5-diethyl diester for homogeneous time-resolved fluorimmunoassay product was synthesized from the raw material diethyl 2,6-dimethylpyridine-3,5-dicarboxylate through N-bromobutanimide(NBS) bromination, electrophilic substitution. The structure and property were confirmed by differential thermal analysis(DTA) Fourier transform infrared spectroscopy(FTIR), proton nuclear magnetic resonance spectroscopy(¹H NMR) and mass spectrometry(MS). Also, the spectral properties of the Eu(Ⅲ)-cryptate chelate, such as absorption spectra, emission spectra, and fluorescence lifetime, were discussed. The fluorescence spectroscopic properties of Eu(Ⅲ)-cryptate are as follows: the excitation peak is at 322 nm, the narrow emission spectrum peaks are at 597 nm(⁵D₀-⁷F₁) and 618 nm(⁵D₀-⁷F₂), respectively, the Stokes shift is 290 nm, the fluorescence lifetime is 918 μs, and the fluorescence quantum yield is Φ=0.249.