核黄素荧光猝灭法测定2,4,6-三硝基苯酚

基于核黄素与2,4,6-三硝基苯酚混合后产生荧光猝灭现象,建立了核黄素作为荧光探针测定2,4,6-三硝基苯酚的新方法。 在0.2 mol/L磷酸盐(NaH₂PO₄-Na₂HPO₄)缓冲溶液(pH=6.2)中,响应时间为1 min时,检测2,4,6-三硝基苯酚的线性范围为2.5~1000 μmol/L,相关系数为0.9938,检测限为0.55 μmol/L。 当加入5.00和20.00 μmol/L 2,4,6-三硝基苯酚到水样后,回收率在98.2%~103.5%之间。 方法简便,选择性好,线性范围宽,可用于实际水样中2,4,6-三硝基苯酚的定性定量分析。

Fluorescent Quenching Method for the Detection of 2,4,6-Trinitrophenol Using Riboflavin

A novel method for the determination of 2,4,6-trinitrophenol has been developed based on fluorescent quenching of riboflavin by 2,4,6-trinitrophenol(TNP) in aqueous solutions. The proposed fluorescent quenching method for TNP detection at pH=6.2 using 0.2 mol/L phosphate(NaH₂PO₄-Na₂HPO₄) as buffer solution responses within 1 min and with a broad linear relationship from 2.5 to 1000 μmol/L(r=0.9938). The limit of detection for TNP is 0.55 μmol/L. When 5.00 and 20.00 μmol/L TNP is added to different water samples, the recovery ranges from 98.2% to 103.5%. Furthermore, this method is simple, selective, and with wide linear range. Therefore, it can be applied in the determination of 2,4,6-trinitrophenol in real samples.