疏水性聚丙烯酸/聚乙烯醇/葡萄糖淀粉酶复合纳米纤维膜的制备及酶学性质

利用混合静电纺丝将葡萄糖淀粉酶(GA)固定于聚丙烯酸(PAA)/聚乙烯醇(PVA)纳米纤维膜上,并通过鉴定固定化GA的酶学特征检验PAA/PVA可否成为一种优良的酶固定化载体。 对其理化性质和酶学特征进行鉴定,经红外光谱(FT-IR)和扫描电子显微镜(SEM)表征发现,GA可成功包埋于PAA/PVA纳米纤维膜内部;对包裹固定的GA进行酶学性质鉴定,发现固定化GA的最适反应温度为68 ℃,比游离GA提高了9 ℃;固定化GA的适用pH值范围明显变宽;热稳定性和存贮稳定性显著增强且可以重复使用。PAA/PVA纳米纤维膜是一种优良的酶固定化载体,可以通过混合静电纺丝包埋法简便地将蛋白质分子固定于其内部,具有一定的应用前景。

Preparation and Enzymatic Characterizations of Hydrophobic Poly(acrylic acid)/Polyvinyl Alcohol/Glucoamylase Composite Nanofiber Membrane

Glucoamylase(GA) was entrapped and immobilized in the poly(acrylic acid)(PAA)/polyvinyl alcohol(PVA) nanofibers membrane by the mixed liquid electrospinning entrapping method. The enzyme immobilization potential of PAA/PVA was investigated through appraised enzymatic characterization of immobilized GA. Its physicochemical properties and enzymatic characterizations were identified. Fourier transform infrared spectrum(FT-IR) and scanning electron microscope(SEM) photographs indicate that GA can be successfully entrapped in the interior of the electrospinning PAA/PVA composite nanofiber membrane. The optimum reaction temperature of immobilized GA is 68 ℃, 9 degrees higher than that of free GA, and the applicable pH range of immobilized GA is wider than that of free GA significantly. In addition, immobilized GA also has good thermal stability, storage stability and reusability. PAA/PVA nanofiber membrane is a good carrier for enzyme immobilization with potential application. The protein molecules can be easily fixed into the inside of nanofiber membrane via mixed liquid electrospinning entrapping method.