| Study of Interaction between Red-tide Toxin, Domoic Acid and Double ——stranded DNA by Capillary Zone Electrophoresis |
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| 作者姓名： | DaZhiLI XinYaHE HuiWANG LiSUN BingChengLIN |
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| 作者单位： | [1]DalianInstituteofChemicalPhysics,ChineseacademyofSciences,Dalian116023 [2]DalianCustomsLaboratory,Dalian116001 |
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| 摘 要： | The interactions between amnesic red-tide toxin, domoic acid (DA) and 14mer double-stranded DNA (dsDNA with three kinds of sequences) were studied by capillary zone electrophoresis (CZE). For the dsDNA with a sequence of 5‘-CCCCCTATACCCGC-3‘, the amount of free dsDNA decreases with the increase of added DA, and the signal of DA-dsDNA complex was observed. Meanwhile, the other two dsDNAs, 5‘-(C)12GC-3‘ and 5‘-(AT)7-3‘, the existence of DA could not lead to the change of dsDNA signal and indicated that there is no interaction between DA and these two dsDNAs.
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| 关 键 词： | 毛细管电泳 双链DNA 赤潮毒素 多摩酸 相互作用 |
Study of Interaction between Red-tide Toxin, Domoic Acid and Double -stranded DNA by Capillary Zone Electrophoresis |
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| DaZhiLI XinYaHE HuiWANG LiSUN BingChengLIN.Study of Interaction between Red-tide Toxin, Domoic Acid and Double -stranded DNA by Capillary Zone Electrophoresis[J].Chinese Chemical Letters,2004,15(9):1079-1082. |
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| Authors: | Da Zhi LI Xin Ya HE Hui WANG Li SUN Bing Cheng LIN Dalian Institute of Chemical Physics Chinese academy of Sciences Dalian Dalian Customs Laboratory Dalian |
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| Institution: | Da Zhi LI1,Xin Ya HE1,Hui WANG1,Li SUN2,Bing Cheng LIN1* 1Dalian Institute of Chemical Physics,Chinese academy of Sciences,Dalian 116023 2Dalian Customs Laboratory,Dalian 116001 |
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| Abstract: | The interactions between amnesic red-tide toxin, domoic acid (DA) and 14mer double-stranded DNA (dsDNA with three kinds of sequences) were studied by capillary zone electrophoresis (CZE). For the dsDNA with a sequence of 5'-CCCCCTATACCCGC-3', the amount of free dsDNA decreases with the increase of added DA; and the signal of DA-dsDNA complex was observed. Meanwhile, the other two dsDNAs, 5'-(C)12GC-3' and 5'-(AT)7-3', the existence of DA could not lead to the change of dsDNA signal and indicated that there is no interaction between DA and these two dsDNAs. |
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| Keywords: | DA dsDNA interaction capillary zone electrophoresis |
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