酶催化反式肉桂酸类似物的不对称氨化反应

在开展应用红酵母PAL酶合成L-苯丙氨酸的基础上,本文首次探讨了该酶不对称氨化反应对部分合成底物的选择性,以拓展它在重要的苯丙氨酸类似物合成方面的应用。

The asymmetric amination of trans-cinnamic acid analogs via enzyme

Investigation on the amination to olefinic bond catalysed by Phenylalanine ammonialyase (PAL, EC 4.3.1.5) contained in Rhodotorula rubra (AS 2.166) whole cells, showed that only the two compounds, i.e. , 3-(2-furyl) acrylic acid (2i), 3-(3-methoxy-4-hydroxyphenyl) acrylic acid (2e), among the eight synthetic analogs of trans-Cinnamic acid: α-methyl-, β-methyl-, 4-nitro-, 4-methoxy-, 4-N, N-dimethylamino-, 4-methyl-Cinnamic acid and 2i, 2e, had been transformed into the corresponding optically pure L-isomer of α-amino acids, i.e., 3-(2- furyl) alanine (3i) and 3-(3-methoxy-4-hydroxyphenyl) alanine (3e). The chemical yields of 3i and 3e were 14.0% and 9.3% respectively, which are lower than that (44.8%) of L-Phenylalanine under the same conditions.