Gd络阴离子进入人红细胞并影响CI^—内流的机制

用质子诱导Ｘ射线发射法测定人红细胞与Ｇｄ柠檬酸和乳酸络合物温育时，胞浆，细胞膜膜脂和膜蛋白上Ｇｄ含量随时间的变化及阴离子通道抑剂对Ｇｄ进入细胞的影响。

Mechanism of Gadolinium Anion Complex Enter into Human Erythrocyte and Its Influence on Chlorion Influx

In order to clarify the mechanism of across-membrane transport of gadollnium complexeswith different charges, the temporal changes of gadolinium content in cytosol, membrane oferythrocytes as well as membrane lipids and membrane proteins were determined by means ofproton induced X-ray emission(PIXE) method during incubation of human erythrocyltes withNa3 Gd(citrate),] and Gd(Lactic)2 ] of. The innuence of anion channel blocker(DIDs) onthe permeability of erythrocytes to gadollnium was investigated. The results show that the anion complexes of gadolinium Penetrate into the cells via anion channels, but the ways other thananion channels cannot be excluded. Gadolinium transPOrt is a process with two stages. In thefirst stage the complexes of gadolinium bind very rapidly to membrane to a large extent. Thenthey are released from the membrane and small amount Penetrates inward to the cytosol slowly.In addtion, the temPOral changes of calcium and chloride content in cytosol were determinedwhen the complexes of gadolinium enter into erythrocytes. The results reveal that the complexes of gadolinium promote chlorion influx, but not calcium. The increase in chlorion influx canbe interpreted by both the "pore formation" and the induced opening of anion channels.