Determination of Anthocyanins by High-Performance Liquid Chromatography: Regularities of Retention

The chromatographic behavior of anthocyanidin derivatives was studied by reversed-phase HPLC. Two types of increment functions in the retention of anthocyanidin glycosides were found. In the first type, an increment corresponding to the replacement of one glycoside with another in a series of different aglycons (as the difference between the logarithms of capacity factors) was independent of the retention of a reference anthocyanin, whereas a linear relationship between the retention parameters was found for the second type.