Liquid chromatographic method for the simultaneous determination of sulphasalazine and its main metabolites in biological fluids |
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| Authors: | K Róna V Winkler T Riesz B Gachályi |
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| Institution: | (1) Department of Medicine, Postgraduate Medical University, I, P.O.B. 112, H-1389 Budapest, Hungary;(2) “A” Department of Rheumatology, National Institute of Rheumatological Disease, P.O.B. 54, H-1525 Budapest, Hungary |
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| Abstract: | Summary An HPLC procedure was developed for the simultaneous determination of salicylazosulphapyridine, and its main metabolites 5-aminosalicylic
acid and sulphapyridine, in human serum and synovial fluid. The analytical procedure consisted of a single ion-pair extraction
step for an Extrelut column with methylene chloride. The investigated compounds and the added sulphadimidine internal standard
were eluated from a Hypersil-MOS reversed-phase column by stepwise gradient; mobile phase was methanol-0.01 M potassium dihydrogenphosphate
(3:7, 0.0–2.0 min and 8:2, 2.1–6.5 min). |
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| Keywords: | Salicylazosulphapyridine 5-aminosalicylic acid Sulphapyridine Rheumatoid arthritis HPLC |
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