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Rapid Screening and Identification of Polar Constituents from Brazilian Arnica Lychnophora sp. by LC-UV/DAD-ESI-MS and LC-UV/DAD-ESI-MS/MS Analysis |
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| Authors: | de Moraes,Solange Leite,Gregó rio,Luiz Elí dio,Tomaz, José Carlos,Lopes, Norberto Peporine |
| Affiliation: | 1.Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café s/n◦, Ribeirão Preto, São Paulo, SP, CEP 14040-903, Brazil ;2.Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes, 3900, Ribeirão Preto, São Paulo, SP, CEP 14040-901, Brazil ; |
| Abstract: | This paper describes an analytical method for the rapid screening and identification of the phenolic constituents present in the polar extracts of different Lychnophora spp. using LC-UV/DAD-ESI-MS and LC-UV/DAD-ESI-MS/MS. Compounds were identified based on UV, retention time, MS experiments and MS/MS of precursor ion or standard. On-line phytochemical investigation of Lychnophora spp. allowed for the identification of flavonoids, chlorogenic acid derivatives and lactones. Some of the observed compounds were for the first time identified in Lychnophora species in a fast analytical procedure. The data obtained here may be helpful to the investigation of polar constituents from other Lychnophora species. |
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