首页 | 本学科首页   官方微博 | 高级检索  
     检索      


Development and Validation of Stability Indicating LC Method to Quantify Captopril in Tablets of Controlled Release
Authors:Stulzer  Hellen Karine  Tagliari  Monika Piazzon  Kuminek  Gislaine  Oliveira  Paulo Renato  Bertol  Charise Dallazen  Silva  Marcos Antonio Segatto
Institution:1.Laboratório de Controle de Qualidade, Departamento de Ciências Farmacêuticas,Universidade Federal de Santa Catarina,Florianópolis,Brazil
Abstract:

An accurate, simple, reproducible, and sensitive liquid chromatographic method was developed and validated for the captopril determination in controlled release tablets. The analyses were performed at room temperature on a reversed-phase Phenomenex Luna C18 column (250 mm × 4.6 mm). The mobile phase was composed of water:methanol (45:55; v/v) pH 2.5, and it was eluted isocratically at a 1.0 mL min−1 flow rate. The method was validated in terms of specificity, linearity, quantification limit, detection limit, accuracy, precision and robustness. The response was linear in the range 0.3–1.5 mg mL−1 (r 2 = 0.9983). The relative standard deviation values for inter-and intra-day precision were 0.77% and 0.50%, respectively. Recoveries ranged between 97.7 and 99.1%. The method was successfully applied for the determination of captopril in the developed formulations.

Keywords:
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号