LC Separation of Fatty Acid Ceramides Using a Two Column System |
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| Authors: | Monika Budvytiene Nataliya Babenko Jolanta Liesiene |
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| Affiliation: | 1.Kaunas University of Technology,Kaunas,Lithuania;2.Institute of Biology,Kharkov Karazin National University,Kharkov,Ukraina |
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| Abstract: | Type II ceramides were separated according to the length of their fatty acid alkyl chain using two column system. The packing of the first column was non-polar adsorbent prepared by coating of macroporous spherical silica with cationized poly(vinyl alcohol) of low substitution degree. Commercial normal phase column Lichrosorb Si 60 was used as a second column in this system. Elution was performed in an isocratic mode using methanol:chloroform 50:50 (v/v) as an eluent. |
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