Exploring Applications of Procerain B,a Novel Protease from <Emphasis Type="Italic">Calotropis procera</Emphasis>, and Characterization by N-Terminal Sequencing as well as Peptide Mass Fingerprinting |
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Authors: | Abhay Narayan Singh Vikash Kumar Dubey |
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Institution: | (1) Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India; |
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Abstract: | Procerain B is a novel cysteine protease isolated from Calotropis procera by our group and published recently. We have further characterized the enzyme by N-terminal sequencing and peptide mass fingerprinting.
Procerain B showed maximum sequence similarity (80%) with Asclepain. Moreover, the characteristic VDWR motif of cysteine proteases
is present in procerain B. The N-terminal and peptide mass fingerprinting analysis showed a distinct nature of the enzyme.
Various applications of the enzyme were also evaluated. Procerain B is very effective in milk-clotting and may be a potential
candidate for this process in the cheese industry. Additionally, the enzyme has potential application as dietary supplement
to aid digestion. Effects of various metal ions on milk-clotting activity were also studied. The milk-clotting activity was
increased in case of few metals while others have a negative effect. It is worth mentioning that the easy availability of
plant material and simple purification method makes industrial production of the enzyme feasible. A protease with easy purification
and suitable properties for application is always desired. |
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