血清游离羟脯氨酸的快速检测

采用6300黄金系统氨基酸分析仪，在锂柱140min程序生理体液分析方法基础上，建立了血清游离羟脯氨酸（HYP）快速测定方法，并用该法检测了正常人及肾病（有或无肾小铧与肾间质纤维化）病人血清中游离HYP的含量；用该法完成分析的时间为24min，比原方法缩短了116min；该法重复性良好，日内RSD2．3％，日间RSD3．7％，回收率97％；纤维化组血清羟脯氨酸浓度显著高于无纤维化组（P＜0．001）和健康对照组（P＜0．001），用该指标显著增高判断肾纤维化，敏感性100％，特异性915，假阳性9％；该法是一种快速、准确、可靠的血清因浆羟脯氨酸定量检测方法，有利于大批量样品的快速测定，并可为临床判断肾病病人肾纤维化提供辅助诊断指标。

Rapid Determination of Hydroxyproline in Sera

A rapid method for the determination of free hydroxyproline (HYP) in sera has been developed with the aid of a high _ performance amino acid analyzer (System 6300). The analytical procedure is based on the 140 _ minute procedure for physiological fluids on a lithium column. The method has been applied to the determination of serum hydroxyproline of healthy blood donors and patients of renal diseases(fibrotic or non _ fibrotic). The analytical time was only 24 min, 116 min shorter than the original method. The relative standard deviation (RSD) within a day was 2.3%, and that between days was 3.7%. The recovery rate was 97%. The serum hydroxy_proline concentrations in the fibrotic group were found to be significantly higher than those in the non _ fibrotic and control groups(P<0.001). An increment in serum hydroxyproline can be used as a parameter for the clinical diagnosis of renal fibrosis. The diagnostic sensitivity, specificity and pseudo positivity of this method were 100%, 91%, and 9%, respectively. The method is rapid, precise and reliable for serum and plasma assays. It is especially useful for large amounts of samples.