Quantifying DNA damage by gel electrophoresis, electronic imaging and number-average length analysis |
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Authors: | Sutherland J C Monteleone D C Trunk J G Bennett P V Sutherland B M |
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Institution: | Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA. jcs@bnl.gov |
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Abstract: | DNA damages that can be converted to single- or double strand breaks can be quantified by separating DNA by gel electrophoresis and obtaining a quantitative image of the resulting distribution of DNA in the gel. We review the theory of this method and discuss its implementation, including the charge-coupled device (CCD) camera systems we developed to acquire images of fluorophore labeled DNA. |
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