Synthesis of Fluorescent Silica Nanoparticles and Their Applications as Fluorescence Probes |
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| Authors: | Xu Song Fang Li Jingwei Ma Nengqin Jia Jianming Xu Hebai Shen |
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| Institution: | (1) Department of Chemistry, Shanghai Normal University, Shanghai, 200234, People’s Republic of China;(2) Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, 200040, People’s Republic of China;(3) Shanghai BaiHuiShen Bio-Sci&Tech Co., Ltd, Shanghai, 200030, People’s Republic of China;(4) Zhongshan Hospital, Fudan University School of Medicine, Shanghai, 200030, People’s Republic of China; |
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| Abstract: | This paper presents the synthesis of organic dye molecules embedded silica nanoparticles by Stöber method and their applications as fluorescence probes in cell imaging. By modifying the surface of fluorescent silica nanoparticles (FSNs) with amino, biologically functionalized and monodisperse FSNs can be obtained. In this work, FSNs were conjugated with monoclonal anti-Carcinoembryonic Antigen (anti-CEA) antibody via covalent binding. The antibody-conjugated FSNs can be used to label the SPCA-1 cells successfully, demonstrating that the application of FSNs as fluorescence probes in fluorescence imaging and bioassay would be feasible. |
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