| 用分光光度法连续测定血清中微量铜、锌的研究 |
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| 引用本文： | 高晓钦,李德芬,谢吉民. 用分光光度法连续测定血清中微量铜、锌的研究[J]. 广东微量元素科学, 1999, 6(7): 44-46 |
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| 作者姓名： | 高晓钦 李德芬 谢吉民 |
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| 作者单位： | [1]镇江医学院无机化学教研室 [2]镇江医学院卫生检验教研室 |
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| 摘 要： | 研究了在ＴｒｉｔｏｎＸ－１００存在下，在ＰＨ８．０的乙醇介质中，用５－Ｂｒ－ＰＡＤＡＰ作为显色剂，应用分光光度法连续测定血清中的铜，锌。本法具有较高的灵敏度，Ｃｕ＾２＋－５－Ｂｒ－ＰＡＤＡＰ的ε为８．５０＊１０＾４Ｌ．ｍｏｌ＾－１．ｃｍ＾－１，Ｚｎ＾２＋－５－Ｂｒ－ＰＡＤＡＰ的ε为９．６５＊１０＾４Ｌ．ｍｏｌ＾－１．ｃｍ＾－１。铜线性范围为０－０．４５ｍｇ．Ｌ＾－１，锌线性范围为０－０．４０ｍｇ．
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| 关 键 词： | 分光光度法 血清 铜 锌 测定 显色剂 |
| 修稿时间： | 1991.1.10) |
Continuous Determination of Trace Element Copperand Zinc in Human Serum by Spectrophotometry |
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| Gao Xiaoqin,Li Defen,Xie Jimin,Zhong Renshan. Continuous Determination of Trace Element Copperand Zinc in Human Serum by Spectrophotometry[J]. Trace Elements Science, 1999, 6(7): 44-46 |
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| Authors: | Gao Xiaoqin Li Defen Xie Jimin Zhong Renshan |
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| Abstract: | A method for continuous determination of trace element copper and zinc in human serum by spectrophotometry with Cu2+-5-Br-PADAP in pH 80 ethanol medium and the presence of Triton X-100 has been established.The sensitivity was good. (Cu2+-5-Br-PADAP) was 850104 Lmol-1cm-1 and (Zn2+-5-Br-PADAP) was 965104 Lmol-1cm-1.The calibration curves were linear in a concentration range of 0045 mgL1 for Cu and 0040 mgL1 for Zn. |
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| Keywords: | Br-PADAP Specrophotometry Serurm Copper Zinc. |
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