脉冲电场作用下Hela细胞穿孔率研究

以宫颈癌Hela细胞为实验对象，利用自制脉冲电源和台盼蓝染色法计数，针对不同的电脉冲参数作用于Hela细胞上，研究了细胞可逆和不可逆电穿孔的场强阈值范围，重点研究了脉冲个数、脉冲宽度和电场强度对细胞不可逆穿孔率的影响，并选择了优化的参数组合。实验发现，在固定脉冲宽度50 μs和20个脉冲个数不变的情况下，Hela细胞出现可逆电穿孔（存活）的场强阈值范围分别为500～750 \begin{document}$ \mathrm{V}/\mathrm{c}\mathrm{m} $\end{document}，出现不可逆电穿孔（死亡）的场强阈值范围为750～1 000 \begin{document}$ \mathrm{V}/\mathrm{c}\mathrm{m} $\end{document}。在对电脉冲参数优化组合的实验中，Hela细胞的不可逆穿孔率随着脉冲个数、脉冲宽度和脉冲场强的增加而增加，最终趋于饱和。宜选择场强1 500 \begin{document}$ \mathrm{V}/\mathrm{c}\mathrm{m} $\end{document}、脉宽60 μs和70个脉冲的参数组合，不可逆穿孔率为93.42%。

Study on perforation rate of Hela cells under pulsed electric field

In view of different electrical parameters on cervical cancer Hela cells, the self-made pulse power supply and trypan blue were used to study the threshold of electric field intensity for reversible and irreversible electroporation of cells. In particular, the influences of the number of pulses, pulse width and electric field intensity on irreversible perforation rate of cells were investigated. The optimal parameter combination was chosen. The experiments results show that the threshold of reversible electroporation (survival) for Hela cells ranges from 500 V/cm to 750 V/cm with a fixed pulse width of 50 μs and a constant number of 20 pulses. Besides, the threshold of irreversible electroporation (death) ranges from 750 V/cm to 1000 V/cm. In the experiment of optimized parameter combination, the irreversible perforation rate of Hela cells increases with the increase of the number of pluses, pulse width and pulse field intensity, and finally reaches saturation. The irreversible perforation rate of Hela cells is 93.42% when the parameter combination of 1500 V/cm, 60 μs and 70 pulses is selected.