Simultaneous Determination of Glucose and Choline Based on the Intrinsic Fluorescence of the Enzymes |
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Authors: | I Sanz-Vicente J J Romero S de Marcos M Ostra C Ubide J Galbán |
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Institution: | (1) GBA, INA, Analytical Chemistry Department, Faculty of Sciences, University of Zaragoza, Pedro Cerbuna 12, 50009 Zaragoza, Spain;(2) GBA, INA, Analytical Chemistry Department, Faculty of Veterinary, University of Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain;(3) Applied Chemistry Department, Faculty of Chemistry, University of País Vasco, Apdo 1072, 20080 San Sebastian, Spain |
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Abstract: | It has been possible to perform the simultaneous determination of choline and glucose using the intrinsic fluorescence of
the corresponding enzyme as an analytical signal. This can be done in two ways. First, for low glucose and choline concentrations
(about 0.55 mM and 0.75 μM respectively) two differentiated signals, without mutual interference, are obtained for both analytes
in the same measurement. Second, when glucose and choline concentrations are higher, a new model has been designed which permits
the concentrations to be accurately determined in samples containing from 0.55 mM to 3.75 mM glucose and from 0.75 μM to 11.0 μM
choline; the method has been applied to simultaneous glucose and choline determinations in serum samples with good results.
This method gives a better performance than multivariate calibration based on Partial Least Squares Regression. The methodology
here shown could be also used for the simultaneous determination of other pairs of analytes.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Intrinsic fluorescence Enzymatic analysis Glucose Choline Simultaneous determination |
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