Ti4+固载的纳米复合硅球的制备及其在磷酸化蛋白分离富集中的应用

采用Staber法,以四乙氧基硅烷(TEOS)和γ-巯丙基三甲氧基硅烷(γ-MPTS)为前驱体合成了硅球纳米粒子,并结合“巯-烯”点击反应和“一锅法”,制备得到固载Ti^4+的纳米复合硅球。通过红外光谱、透射电镜等方法对材料进行表征,利用蛋白吸附实验以及凝胶电泳等方法探究了该亲和材料对磷酸化蛋白的分离富集效果。结果表明,该纳米复合材料分散性好,粒径均一,对磷酸化蛋白(α-酪蛋白)的最大吸附容量(12.27μmol/g)远大于非磷酸化蛋白(辣根过氧化物酶,1.12μmol/g),且能从牛奶实际样品中分离富集磷酸化蛋白。

Preparation of Ti4+ Immobilized Silica Nanocomposites and Their Application in Separation and Enrichment of Phosphoproteins

The Ti4+immobilized silica nanocomposites(SiO2-Ti4+)were fabricated by synthesizing silica nanoparticles with tetraethoxysilane(TEOS)andγ-mercaptopropyl trimethoxysilane(γ-MPTS)as precursors,followed by"thiol-ene"click reaction and"one-pot"process.The morphology and structure properties of the resultant SiO2-Ti^4+were characterized by transmission electronic microscopy(TEM),Fourier transform infrared spectroscopy(FT-IR)and X-ray photoelectron spectroscopy(XPS).The adsorption behavior of the SiO2-Ti^4+towards phosphoproteins was investigated.Under the optimized conditions,the SiO2-Ti^4+showed a rather higher adsorption capacity towards phosphoproteins(α-casein,12.27μmol/g)than non-phosphoproteins(horseradishperoxidase,1.12μmol/g).In addition,the practicality of the SiO2-Ti^4+was further demonstrated by their selective enrichment of phosphoproteins from milk samples.