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| 黑斑蛙早期胚胎发育过程中蛋白酶活性 | |
| 引用本文： | 张文学,张新胜,薛德明,马茹,马全祥,徐存拴.黑斑蛙早期胚胎发育过程中蛋白酶活性[J].河南师范大学学报(自然科学版),1999,27(4):54-57. |
| 作者姓名： | 张文学 张新胜 薛德明 马茹 马全祥 徐存拴 |
| 作者单位： | 1. 河南师范大学生命科学学院,河南新乡,453002 2. 新乡市中心医院 3. 新乡医学院 |
| 基金项目： | 河南省动物学重点学科基金，河南省教委自然科学基金 |
| 摘 要： | 本文采用活性电泳方法，对黑斑蛙（Ｒａｎａ ｎｉｇｒｏｍ ａｃｕｌａｔａ）早期胚胎发育过程中蛋白酶活性进行了研究．结果如下：尾芽期以前未检测到蛋白酶活性；中性条件下，心跳期检出分子量分别为８０、７２、６６ｋＤ的三种蛋白酶；随着胚胎的发育，蛋白酶的种类逐渐增多、活性逐渐增强；三种ｐＨ 条件下各时期所检出的酶的种类及活性并不相同，但以中性条件下酶的种类多、活性强． |
| 关 键 词： | 黑斑蛙 早期胚胎发育 蛋白酶 活性电泳(G-PAGE) |
| 文章编号： | 1000-2367(1999)04-0054-04 |
| 修稿时间： | 1998-10-26 |
The Proteinase Activity During Early Embryonic Development of Rana nigromaculata |
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| ZHANG Wen-xue,ZHANG Xin-sheng,XUE De-ming,MA Ru,MA Quan-xiang,XU Cun-xiang.The Proteinase Activity During Early Embryonic Development of Rana nigromaculata[J].Journal of Henan Normal University(Natural Science),1999,27(4):54-57. | |
| Authors: | ZHANG Wen-xue ZHANG Xin-sheng XUE De-ming MA Ru MA Quan-xiang XU Cun-xiang |
| Abstract: | The G-PAGE was used to analyze the proteinase activity of Rana nigromaculata during early embryonic development. The results showed that: ①Before tail bud stage, there was almost no proteinase activity;②At heart beat stage, three kinds of different proteinase 85, 72 and 66kD appeared; ③The activity and the sorts of proteinase increased gradually in the course of embryonic development.④Under three pH conditions, the activity and the sorts of proteinase was different. The hightest was at pH 7.0. |
| Keywords: | rana nigromaculata early embryonic development proteinase G-PAGE(Gelatine polyacrylamide gels electrophoresis) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |