Determination of cimetidine in human plasma by use of coupled-flow injection, solid-phase extraction, and capillary zone electrophoresis |
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Authors: | J -W Luo H -W Chen Q -H He |
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Institution: | (1) Chemistry Department, Zhejiang University, Xixi Campus, 310028 Hangzhou, China;(2) Present address: Zhejiang Provincial Institute for Drug Control, 310004 Hangzhou, China |
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Abstract: | Summary An on-line flow injection-solid-phase extraction-capillary zone electrophoresis (FI-SPE-CZE) method has been developed for
determination of cimetidine in human plasma. Sodium dodecylsulfate (SDS) was used as dynamic chemical modifier for elimination
of capillary contamination by biological macromolecules. FI on-line preconcentration and cleaning of the analyte by means
of a C18 microcolumn was performed automatically and CZE separation was performed consecutively without interruption of the applied
voltage and between-run-washing of the capillary. A detection limit of 8 μgL−1 (3×σ) was achieved at a sample throughput of 12h−1. The approach was successfully used for a pharmacokinetic study of cimetidine. |
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Keywords: | Capillary electrophoresis Solid-phase extraction Flow-injection preconcentration Cimetidine Plasma |
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