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Preparation of Porous Protein-Based Hydogel for Highly Sensitive Protein Chips |
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| Authors: | Hiroyuki Tanaka Toshifumi Shiroya Minako Hanasaki Tatsushi Isojima Hisao Takeuchi Haruma Kawaguchi |
| Institution: | 1. Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama Japan
Mitsubishi Chemical Group Science and Technology Research Center, INC 1000 Kamoshida-cho, Aoba-ku, Yokohama Japan;2. Mitsubishi Chemical Group Science and Technology Research Center, INC 1000 Kamoshida-cho, Aoba-ku, Yokohama Japan;3. Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama Japan |
| Abstract: | Summary: Protein chips are important tools for high-throughput analysis of biological events. We have developed a novel method to prepare a protein-based hydrogel, that is, a “Three-Dimensional Nano-structured Protein Hydrogel” (3-D NPH), which is composed of protein and polymer nano-particles. The 3-D NPH could be easily prepared by dispensing a protein and polymer mixture on a substrate. Surprisingly, gold particles conjugated with protein A diffused into the 3-D NPH which was made of mouse IgG through the pores. We have shown that the protein chips made with our 3-D NPH method has tremendously improved sensitivity in detecting protein-protein interactions compared with that of direct protein immobilization methods. |
| Keywords: | high sensitivity hydrogel nano-particle protein chip protein immobilization |