多元线性模型计算基于荧光猝灭原理的膜传感器对分析物的响应参数

根据基于荧光猝灭原理的化学传感膜的结构特征和发光机理,推导并提出量化作用于膜传感器的多种荧光猝灭因素的多元线性模型.结合三杯法,该模型可简便地求出反映不同荧光猝灭机理的特征参数.通过建立的数学模型,测定并计算了芘丁酸膜传感器对应于呋喃妥因等23种药物多种荧光猝灭因素的响应数据和响应参数.结果表明,传感膜对分析物的响应特征往往能得到主要猝灭因素响应模型的近似反映,这为基于荧光猝灭原理的化学传感器定量分析模型的建立提供了可借鉴的思路和方法.

A Multivariate Linear Model for Calculating Response Feature Parameters of Chemical Sensor Based on Fluorescence Quenching for Analytes

A mathematical model was deduced and described for the different mechanism of fluorescence quenching to analytes based on the essential quenching models including the dynamic quenching, the resonance energy transfer and the inner filter effects involving absorption of both excited light from the light source and emitted light from membrane. The model reported here indicated that the logarithmic functions of the intensity of fluorescence modulation in virtue of multiple quenching factors conform to the rule of addition. The response feature parameters of chemical sensor based on fluorescence quenching for analyses could be calculated by combining the multivariate linear model with a tri cup method. A conception based on regarding the chemical sensor as the second source of light was advanced to get more useful information on analytes. The pyrenebutyric acid was chosen as a fluoroprobe for constructing the chemical sensor to response a series of the therapeutic drugs. A train of thought for constructing the quantitative analysis model for the chemical membrane sensors based on the fluorescence quenching was afforded in the light of the experimental results.