Chromatographic aspects in high throughput liquid chromatography/mass spectrometry |
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Authors: | Pereira L Ross P Woodruff M |
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Affiliation: | ThermoQuest Chromatography Supplies, Hypersil Division, 112 Chadwick Road, Astmoor, Runcorn, Cheshire WA7 1PR, UK. |
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Abstract: | Important parameters to consider when developing a liquid chromatography/mass spectrometry (LC/MS) method are buffer type and concentration, and column geometry. In the work presented here the choice of buffer for the analysis of basic compounds using a polar embedded phase (HyPURITYtrade mark ADVANCE) is illustrated for the analysis of tricyclic antidepressants. Method transfer from a 4.6 mm i.d. column to a 2.1 mm i.d. column is demonstrated for the analysis of triazines and anabolic steroids and their metabolites, with no change in selectivity and with added speed of analysis. Analysis of eight beta-blockers is achieved in 65 seconds by using a short 30 x 4.6 mm C18 column. |
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