Thermoresponsive and bioactive poly(vinyl ether)-based hydrogels synthesized by radiation copolymerization and photochemical immobilization |
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| Affiliation: | 1. Chemical Engineering Department, Hacettepe University, 06800 Beytepe, Ankara, Turkey;2. Laboratory for Molecular Surfaces and Nanotechnology (LAMSUN), Dipartimento di Scienze Chimiche, University of Catania, Italy;3. Consorzio Interuniversitario per i Sistemi a Grande Interfaccia (CSGI), V. le A. Doria 6, 95125 Catania, Italy;1. Key Laboratory of Theoretical Chemistry of Environment, Ministry of Education, Department of Chemistry, South China Normal University, Guangzhou 510006, China;2. Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China;3. Guangdong Key Laboratory of Green Processing of Natural Products and Product Safety, School of Light Industry and Food Science, South China University of Technology, Guangzhou 510641, China;1. State Key Lab of Chemical Resource Engineering, College of Science, Beijing University of Chemical Technology, Beijing 100029, People’s Republic of China;2. Department of Organic Chemistry, College of Science, Beijing University of Chemical Technology, Beijing 100029, People’s Republic of China;1. Center for Electronic Materials, Korea Institute of Science and Technology (KIST), Seoul 136-791, Republic of Korea;2. Department of Nanomaterials Science and Technology, Korea University of Science and Technology, Daejeon 305-333, Republic of Korea |
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| Abstract: | A thermoresponsive hydrogel was synthesized by radiation copolymerization of ethylene glycol vinyl ether (EGVE) and butyl vinyl ether (BVE) in the presence of cross-linking agent diethylene glycol divinyl ether. The gel was modified by a cell adhesion factor RGD by photochemical immobilization technique. While the unmodified hydrogel shows fully hydrated form at low temperatures (+4 °C) and it extensively dehydrates at 37 °C, the biomodified hydrogel still kept its thermoresponsive character after immobilization. The effectiveness of immobilization was checked with FTIR-ATR and XPS. The use of bioactive thermoresponsive hydrogels in cell culture applications was investigated. For this purpose, cell culture experiments were realized by L929 mouse fibroblasts. Cell attachment experiments revealed the effect of immobilized RGD with higher values of cell attachment (∼85%), which were obtained especially in the absence of serum. The thermoresponsive character of the hydrogel was useful for the application of low-temperature treatment in order to recover the attached viable cells from the surface of the hydrogel without using trypsin. When the culture temperature was decreased from 37 to 10 °C for 30 min ∼80% of the cells were detached from the hydrogel surface. |
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