| ELECTROFUSION OF IBRS2 CELLS AND THE STUDY OF THEIR FUSION PROCESS |
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| 作者姓名： | 郑强 赵南明 |
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| 作者单位： | Department of Biological Sciences and Biotechnology，Tsinghua University，Beijing，Department of Biological Sciences and Biotechnology，Tsinghua University，Beijing |
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| 摘 要： | IBRS2 epithelial cells in monolayer culture fused at a very high frequency when exposed to high-voltage electric pulsing fields. Exposure to four repetitive electric pulses of about 1.7 kilovolts per centimeter with a duration of 100 microseconds caused more than 90 percent of the cells to become fused (multinucleate) when 1 millimolar magnesium was present in the pulsing medium. Magnesium and calcium ions in the pulsing medium had a very strong effect on the electrofusion of IBRS2 cells. Magnesium could increase not only the electrofusion yield but also the stability of the cells under the conditions of electrofusion. In contrast, calcium inhibited electrofusion and decreased the stability of the cells. Careful microscopic observation revealed the electrofusion of IBRS2 cells to be very complex, dynamic process undergoing many interesting changes. A possible explanation for the process and mechanism of electrofusion of IBRS2 cells was proposed in agreement with the experimental observation.
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ELECTROFUSION OF IBRS2 CELLS AND THE STUDY OF THEIR FUSION PROCESS |
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| ZHENG QIANG AND ZHAO NAN-MING.ELECTROFUSION OF IBRS2 CELLS AND THE STUDY OF THEIR FUSION PROCESS[J].Science in China(Chemistry),1989(3). |
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| Authors: | ZHENG QIANG AND ZHAO NAN-MING |
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| Abstract: | IBRS2 epithelial cells in monolayer culture fused at a very high frequency when exposed to high-voltage electric pulsing fields. Exposure to four repetitive electric pulses of about 1.7 kilovolts per centimeter with a duration of 100 microseconds caused more than 90 percent of the cells to become fused (multinucleate) when 1 millimolar magnesium was present in the pulsing medium. Magnesium and calcium ions in the pulsing medium had a very strong effect on the electrofusion of IBRS2 cells. Magnesium could increase not only the electrofusion yield but also the stability of the cells under the conditions of electrofusion. In contrast, calcium inhibited electrofusion and decreased the stability of the cells. Careful microscopic observation revealed the electrofusion of IBRS2 cells to be very complex, dynamic process undergoing many interesting changes. A possible explanation for the process and mechanism of electrofusion of IBRS2 cells was proposed in agreement with the experimental observation. |
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| Keywords: | electrofusion IBRS2 cells fusion process |
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