毛细管电泳高频电导法测定几种中药中的丁香酚

在三乙胺的弱碱性体系中, 对几种不同产地中药中的丁香酚含量进行了测定, 结果显示这几种中药中的丁香酚含量存在明显的地区差异.

Determination of Eugenol in Several Chinese Herbal Medicines by Capillary Electrophoresis with High Frequency Conductivity Method

A method based on capillary electrophoresis with a high frequency conductivity method was developed for the determination of eugenol in several Chinese herbal medicines from different producing areas. In this study, some key factors for separation and detection were studied and the best analysis conditions were obtained. In the buffer system of 2.0 mmol/L triethylamine +0.74 mol/L methanol at separation potential of 18.0 kV, the optimal separation and detection wasobtained. Under the chosen conditions, eugenol was eluted within 7 min. The linear range of eugenol was 2.0—540 μg/mL, the limit of detection was 0.6 μg/mL, and the recovery ranged from 93.3% to 106%.