Measuring calcium uptake and inositol 1,4,5-trisphosphate-induced calcium release in cerebellum microsomes using Fluo-3 |
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| Authors: | Francesco Michelangeli |
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| Institution: | (1) School of Biochemistry, University of Birmingham, Edgbaston, B15 2TT Birmingham, UK |
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| Abstract: | A simple fluorimetric method is presented for measuring calcium uptake and inositol 1,4,5-trisphosphate (InsP3)-induced calcium release in microsomes using Fluo-3. This method is used to investigate the amplitude of calcium release (quantal release) induced by InsP3 from rat cerebellum microsomes. |
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| Keywords: | Fluo-3 fluorescence calcium inositol 1 4 5-trisphosphate |
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