SEPARATION AND PHOTOTOXICITY IN VITRO OF SOME OF THE COMPONENTS OF HAEMATOPORPHYRIN DERIVATIVE |
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| Authors: | P A Cowled I J Forbes A G Swincer V C Trenerry A D Ward |
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| Institution: | Department of Medicine, The University of Adelaide, The Queen Elizabeth Hospital, Woodville, Australia 5011;Department of Organic Chemistry, The University of Adelaide, Adelaide, Australia 5000 |
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| Abstract: | Abstract— The separation and fractionation of some of the components of haematoporphyrin derivative by chemical extraction, gel chromatography and membrane filtration are described. The composition of the various fractions and the purity of the individual porphyrins can be assessed by high performance liquid chromatography. Porphyrins containing vinyl side chains show an unusual ability to form relatively stable aggregates of apparently high molecular weight. The effect is most pronounced with protoporphyrin and is not observed with haematoporphyrin which has no vinyl side chains. These aggregates can be separated by gel chromatography or membrane filtration from non (or less) aggregated material. The aggregates show greater in vitro cytotoxicity than the other fractions. |
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