HPLC‐PAD‐atmospheric pressure chemical ionization‐MS metabolite profiling of cytotoxic carotenoids from the echinoderm Marthasterias glacialis (spiny sea‐star) |
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| Authors: | Federico Ferreres David M Pereira Angel Gil‐Izquierdo Patrícia Valentão João Botelho Teresa Mouga Paula B Andrade |
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| Institution: | 1. Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), Murcia, Spain;2. REQUIMTE/Department of Pharmacognosy, Faculty of Pharmacy, Porto University, Porto, Portugal;3. GIRM – Marine Resources Research Group, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, Santuário Na. Sra. Dos Remédios, Peniche, Portugal |
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| Abstract: | An HPLC‐PAD‐atmospheric pressure chemical ionization‐MS metabolite profiling analysis was conducted on the marine echinoderm Marthasterias glacialis (spiny sea‐star). Bio‐guided purification of the methanolic extract led to the isolation of several carotenoids, namely zeaxanthin, astaxanthin and lutein. These compounds were characterized using both UV–Vis characteristics and MS spectra interpretation. No previous works addressed the MS analysis of carotenoids present in this organism. The purified carotenoid fraction displayed a strong cell proliferation inhibition against rat basophilic leukemia RBL‐2H3 (IC25=268 μg/mL) cancer cell line. Against healthy V79 (rat lung fibroblasts (IC25=411 μg/mL)) cell line, however, toxicity was lower, as it is desired for anti‐cancer molecules. This study suggests that M. glacialis may constitute a good source of bioactive compounds that can be used as lead compounds for the pharmaceutical industry. |
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| Keywords: | Carotenoids Cytotoxic HPLC‐PAD‐atmospheric pressure chemical ionization‐MS Marthasterias glacialis |
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