Effect of immobilization of polysaccharides on the biocompatibility of poly(butyleneadipate‐co‐terephthalate) films |
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Authors: | Win‐Chun Jao Chien‐Hong Lin Jui‐Yuan Hsieh Yi‐Hsing Yeh Chia‐Yi Liu Ming‐Chien Yang |
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Institution: | 1. Department of Polymer Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan, ROC;2. Department of Biological Science and Technology, China Institute of Technology, Taipei 11581, Taiwan, ROC |
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Abstract: | Aiming to improve the hydrophilicity, antibacterial activity, cytocompatibility, and hemocompatibility of poly(butyleneadipate‐co‐terephthalate) (PBAT) films, PBAT films were treated with ozone, grafted with chitosan (CS), and followed by covalent immobilization of either heparin (HEP) or hyaluronic acid (HA). The surface graft density of modified PBAT films was detected by X‐ray photoelectron spectroscopy (XPS) and dyeing. The surface roughness of PBAT films was measured using an atomic force microscope (AFM). After immobilizing CS, PBAT films acquired antibacterial activity against Staphylococcus aureus and Escherichia coli. The adsorption of human serum albumin (HSA) and human plasma fibrinogen (HPF) on PBAT–CS–HEP and PBAT–CS–HA films was lower compared to that of native PBAT. Moreover, HEP immobilization could effectively reduce platelet adhesion and prolong the blood coagulation time, thereby improving the blood compatibility of PBAT. In addition, the growth of L929 fibroblasts was improved for HEP or HA immobilized PBAT, suggesting this surface modification was non‐cytotoxic. Furthermore, PBAT–CS–HEP and PBAT–CS–HA exhibited higher cell proliferation than native PBAT. Copyright © 2009 John Wiley & Sons, Ltd. |
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Keywords: | poly(butyleneadipate‐co‐terephthalate) chitosan heparin hyaluronic acid antibacterial activity hemocompatibility cytocompatibility |
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