Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto‐Daigaku‐Katsura, Nishikyo‐ku, Kyoto 615‐8510, Japan
Abstract:
A genetically modified His‐tagged endoglucanase, EGIIcore2, with two active sites in series was immobilized on gold via three different kinds of anchor molecules, and its hydrolytic activity was studied. Immobilization of EGIIcore2 was influenced by the chain length and hydrophilicity of anchor molecules. The hydrolytic activity of the immobilized EGIIcore2 was nearly the same on either anchor molecule. Interestingly, the immobilized EGIIcore2 apparently retained the inherent hydrolytic activity similarly to free EGIIcore2. It is therefore considered that the local high concentration of EGIIcore2 on the surface should promote the successive hydrolysis of the transient products to show the high hydrolytic activity despite of immobilization.
