A new open tubular capillary microextraction and sweeping for the analysis of super low concentration of hydrophobic compounds |
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| Authors: | Zhining Xia Tingting Gan Hua Chen Rui Lv Weili Wei Fengqing Yang |
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| Institution: | 1. College of Bioengineering, Chongqing University, Chongqing, P. R. China;2. College of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P. R. China;3. State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco‐Environmental Sciences, Chinese Academy of Sciences, Beijing, P. R. China |
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| Abstract: | A sample pre‐concentration method based on the in‐line coupling of in‐tube solid‐phase microextraction and electrophoretic sweeping was developed for the analysis of hydrophobic compounds. The sample pre‐concentration and electrophoretic separation processes were simply and sequentially carried out with a (35%‐phenyl)‐methylpolysiloxane‐coated capillary. The developed method was validated and applied to enrich and separate several pharmaceuticals including loratadine, indomethacin, ibuprofen and doxazosin. Several parameters of microextration were investigated such as temperature, pH and eluant. And the concentration of microemulsion that influences separation efficiency and microextraction efficiency were also studied. Central composite design was applied for the optimization of sampling flow rate and sampling time that interact in a very complex way with each other. The precision, sensitivity and recovery of the method were investigated. Under the optimal conditions, the maximum enrichment factors for loratadine, indomethacin, ibuprofen and doxazosin in aqueous solutions are 1355, 571, 523 and 318, respectively. In addition, the developed method was applied to determine loratadine in rabbit blood sample. |
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| Keywords: | Hydrophobic compounds In‐line coupling In‐tube solid‐phase microextraction Sample pre‐concentration Sweeping |
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