A simple methodology for the determination of fatty acid composition in edible oils through 1H NMR spectroscopy |
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| Authors: | Andersson Barison Caroline Werner Pereira da Silva Francinete Ramos Campos Fabio Simonelli Cesar Antonio Lenz Antonio Gilberto Ferreira |
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| Institution: | 1. Laboratório de Ressonancia Magnética Nuclear—Departamento de Química, Centro Politécnico, Universidade Federal do Paraná (UFPR), C.P. 19081, CEP 81530‐900, Curitiba, PR, Brazil;2. Instituto de Tecnologia do Paraná (TECPAR), R. Prof. Algacyr Munhoz Mader, 3775, CIC, C.P. 357, CEP 81350‐010, Curitiba, PR, Brazil;3. Laboratório de Ressonancia Magnética Nuclear—Departamento de Química, Universidade Federal de S?o Carlos (UFSCar), Via Washington Luís, Km 235, C.P. 676, CEP 13565‐905, S?o Carlos, SP, Brazil |
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| Abstract: | A simple methodology for the determination of the fatty acid composition of edible oils through 1H NMR is proposed. The method is based on the fact that all fatty acid chains are esterified to a common moiety, glycerol, and the quantification is done directly in the 1H NMR spectra through the relationship between the areas of a characteristic signal of each fatty acid and a signal of the glycerol moiety, without the use of mathematical equations. The methodology was successfully applied to determine the fatty acid composition of several edible oils, with equivalent results to those given by the AOAC Official method by gas chromatography. Its main advantages are simplicity and the lack of need for sample pre‐treatment such as derivatization or extraction. Copyright © 2010 John Wiley & Sons, Ltd. |
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| Keywords: | NMR 1H NMR fatty acid fatty acid composition edible oil vegetable oil triacylglycerol |
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