Analysis and tentative structure elucidation of new anthocyanins in fruit peel of Vitis coignetiae Pulliat (meoru) using LC‐MS/MS: Contribution to the overall antioxidant activity |
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Authors: | Jin Young Choi Soo Jung Lee Sung Joong Lee Semin Park Jung Han Lee Jae‐Han Shim A M Abd El‐Aty Jong‐Sung Jin Euh‐Duck Jeong Won Sup Lee Sung Chul Shin |
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Institution: | 1. Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju, Republic of Korea;2. Department of Food and Nutrition, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Republic of Korea;3. Natural Products Chemistry Laboratory, Institute of Agricultural Science and Technology, College of Agriculture and Life Science, Chonnam National University, Buk‐Ku, Gwangju, Republic of Korea;4. Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt;5. Korea Basic Science Institute Busan Center, High Technique Components & Materials Team, Busan, Republic of Korea;6. Department of Internal Medicine, Institute of Health Sciences, and Gyeongnam Regional Cancer Center, Gyeongsang National University, Jinju, Republic of Korea |
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Abstract: | The skin of Vitis coignetiae Pulliat (meoru) grown wild in the Republic of Korea was analyzed for anthocyanins via HPLC coupled to ESI‐MS/MS in positive ion mode. Chromatographic separation was conducted via RP HPLC using a C18 column, with a 50‐min gradient from 0 to 80% methanol in water containing 0.5% formic acid. A total of 18 anthocyanins were identified. Among them, nine compounds were newly determined by comparing the retention time (tR) and mass fragmentation patterns with those of the previously reported anthocyanins for other grape varieties: malvidin hexose, peonidin 3‐galactoside, malvidin 3‐galactoside, cyanidin, petunidin, petunidin 3‐(6″‐coumaroyl)‐5‐diglucoside, peonidin, malvidin, and malvidin 3‐(6″‐coumaroyl)‐5‐diglucoside. The antioxidant activity of the V. coignetiae Pulliat anthocyanins was determined via 2,2‐diphenyl‐2‐picrylhydrazyl radical scavenging and 2,2′‐azinobis‐(3‐ethylbenzothiazoline‐6‐sulfonic acid) radical cation assays in a range of concentration from 25 to 500 mg/L. The capacity increased with concentration. The IC50 values, defined as the concentration of sample required to scavenge 50% of free radicals, were calculated as follows: 189.63±11.31 mg/L and 141.29±6.70 mg/L for 2,2‐diphenyl‐2‐picrylhydrazyl and 2,2′‐azinobis‐(3‐ethylbenzothiazoline‐6‐sulfonic acid) radical cation, respectively. The antioxidant activity of the V. coignetiae Pulliat anthocyanins is substantially higher than that of ascorbic acid and is similar to the effects of the extracts obtained from other grape varieties. |
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Keywords: | Anthocyanins Antioxidant effect LC MS/MS Vitis coignetiae Pulliat |
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