Distribution and mobility of phosphates and sodium ions in cheese by solid‐state 31P and double‐quantum filtered 23Na NMR spectroscopy |
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Authors: | Mallory Gobet Corinne Rondeau‐Mouro Solange Buchin Jean‐Luc Le Quéré Elisabeth Guichard Loïc Foucat Céline Moreau |
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Affiliation: | 1. INRA, ENESAD, Université de Bourgogne, UMR1129 FLAVIC, F‐21000 Dijon, France;2. INRA, UR 1268 BIA, Plate‐forme BIBS, F‐44300 Nantes, France;3. INRA, UR 342 Technologie et Analyses Laitières, F‐39800 Poligny, France;4. INRA, UR370 QuaPA, F‐63122 Saint Genès Champanelle, France |
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Abstract: | The feasibility of solid‐state magic angle spinning (MAS) 31P nuclear magnetic resonance (NMR) spectroscopy and 23Na NMR spectroscopy to investigate both phosphates and Na+ ions distribution in semi‐hard cheeses in a non‐destructive way was studied. Two semi‐hard cheeses of known composition were made with two different salt contents. 31P Single‐pulse excitation and cross‐polarization MAS experiments allowed, for the first time, the identification and quantification of soluble and insoluble phosphates in the cheeses. The presence of a relatively ‘mobile’ fraction of colloidal phosphates was evidenced. The detection by 23Na single‐quantum NMR experiments of all the sodium ions in the cheeses was validated. The presence of a fraction of ‘bound’ sodium ions was evidenced by 23Na double‐quantum filtered NMR experiments. We demonstrated that NMR is a suitable tool to investigate both phosphates and Na+ ions distributions in cheeses. The impact of the sodium content on the various phosphorus forms distribution was discussed and results demonstrated that NMR would be an important tool for the cheese industry for the processes controls. Copyright © 2010 John Wiley & Sons, Ltd. |
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Keywords: | NMR 23Na 31P double‐quantum filter magic angle spinning solid state bound sodium ions phosphate distribution semi‐hard cheese |
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