Compensating for variation in 1JCH coupling constants in HSQC spectra acquired on small organic molecules |
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Authors: | Peter W. A. Howe |
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Affiliation: | Analytical Sciences, Syngenta, Jealott's Hill Research Centre, Bracknell RG42 6EY, UK |
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Abstract: | The HSQC sequence provides a sensitive way of determining the 13C chemical shift of protonated carbons. It uses INEPT elements for magnetization transfer, which can only be optimized for one value of 1JCH, but small organic molecules contain a wide range of 1JCH values. One popular method of compensating for 1JCH variation is to incorporate adiabatic pulses into the INEPT elements. This article shows that this method fails for a significant subset of functional groups. It also shows that the effects of this failure can be reduced by avoiding refocusing delays and by using a J‐compensated excitation element. Copyright © 2010 John Wiley & Sons, Ltd. |
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Keywords: | NMR 1H 13C 1JCH HSQC INEPT J‐compensation |
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