Development of a tandem time‐of‐flight mass spectrometer with an electrospray ionization ion source |
| |
| Authors: | Hirofumi Nagao Shuichi Shimma Shigeo Hayakawa Kunio Awazu Michisato Toyoda |
| |
| Affiliation: | 1. Renovation Center of Instruments for Science Education and Technology, Osaka University, 1‐1 Machikaneyama, Toyonaka, Osaka 560‐0043, Japan;2. Venture Business Laboratory, Center for Advanced Science and Innovation, Osaka University, 2‐1 Yamadaoka, Suita, Osaka 565‐0871, Japan;3. Department of Chemistry, Graduate School of Science, Osaka Prefecture University, 1‐1 Gakuencho, Nakaku, Sakai, Osaka 599‐8531, Japan;4. Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University, 2‐1 Yamadaoka, Suita, Osaka 565‐0871, Japan;5. Department of Physics, Graduate School of Science, Osaka University, 1‐1 Machikaneyama, Toyonaka, Osaka 560‐0043, Japan |
| |
| Abstract: | A new tandem time‐of‐flight mass spectrometer with an electrospray ionization ion source ‘ESI‐TOF/quadTOF’ was designed and constructed to achieve the desired aim of structural elucidation via high‐energy collision‐induced dissociation (CID), and the simultaneous detection of all fragment ions. The instrument consists of an orthogonal acceleration‐type ESI ion source, a linear TOF mass spectrometer, a collision cell, a quadratic‐field ion mirror and a microchannel plate detector. High‐energy CID spectra of doubly protonated angiotensin II and bradykinin were obtained. Several fragment ions such as a‐, d‐, v‐ and w‐type ions, characteristic of high‐energy CID, were clearly observed in these spectra. These high‐energy CID fragment ions enabled confirmation of the complete sequence, including leucine–isoleucine determinations. It was demonstrated that high‐energy CID of multiply protonated peptides could be achieved in the ESI‐TOF/quadTOF. Copyright © 2010 John Wiley & Sons, Ltd. |
| |
| Keywords: | tandem time‐of‐flight mass spectrometer ESI‐TOF/quadTOF multiply protonated peptide high‐energy CID quadratic‐field ion mirror |
|
|
