Simultaneous determination of substrate and product in the process of preparation of valienamine by capillary zone electrophoresis |
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Authors: | Xiao‐Dong Wei Wen‐Jie Zhao Min Gu Bo Zhao Rong‐Ying Yao |
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Institution: | 1. Shanghai Institute of Pharmaceutical Industry, Shanghai, P. R. China;2. Pucheng Lifecome Biochemistry Co., Ltd., Fujian, P. R. China |
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Abstract: | A simple and rapid CZE method was established for the simultaneous determination of valienamine, acarbose and validamycin A, using a 20‐kV CZE with the detection wavelength of 193 nm and 50 mM phosphoric acid–20 mM Tris (pH 5.3) as a running buffer. The calibration curves of valienamine, acarbose, and validamycin A showed a good linear relationship at a concentration range of 5–1000 μg/mL. The detection limits of valienamine, acarbose, and validamycin A were 0.3, 0.6, and 0.6 μg/mL, respectively, and the average recoveries of each of the above were 99.9, 99.5, and 100.3%. The method has been successfully applied for simultaneous determination of substrate and product in the process of preparation of valienamine. |
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Keywords: | Acarbose Capillary zone electrophoresis Validamycin A Valienamine |
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